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propidium iodide pi  (Elabscience Biotechnology)


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    Elabscience Biotechnology propidium iodide pi
    Propidium Iodide Pi, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/propidium+iodide+pi+solutions/Propidium+Iodide+(PI)+Solution/pmc13100712-267-50-66
    Average 95 stars, based on 148 article reviews
    propidium iodide pi - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: SNAPIN Facilitates Progression of Hepatocellular Carcinoma by Hindering Ferroptosis Through KEAP1 Degradation Promotion
    Article Snippet: .. Following a single wash of the cells in basal medium, a 30 min incubation with calcein AM and propidium iodide (PI) solutions (Elabscience, China) proceeded at ambient temperature. .. Subsequently, images were captured by fluorescent microscopy (Olympus, Tokyo, Japan).

    Article Title: SNAPIN Facilitates Progression of Hepatocellular Carcinoma by Hindering Ferroptosis Through KEAP1 Degradation Promotion.
    Article Snippet: .. Following a single wash of the cells in basal medium, a 30 min incubation with calcein AM and propidium iodide (PI) solutions (Elabscience, China) proceeded at ambient temperature. .. Subsequently, images were captured by fluorescent microscopy (Olympus, Tokyo, Japan).



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    Dynamic suspension culture using mixed of chitosan and chitin nanofiber scaffolds. Comparison of agitation conditions in the presence of chitosan/chitin (80:20) using a 30-mL bioreactor. (A) Specific growth rate ( μ ) during 24–96 h. Data are mean ± s.d.; no significant difference was detected (Tukey’s test, n = 3 per culture). (B) Distribution of aggregate projected area at t = 96 h. Comparison of scaffold conditions at 50 rpm in a 30-mL bioreactor. (C) Growth profiles under without scaffolds (open triangle, △), chitin only (open circle, ○), and chitosan/chitin (80:20) (closed circle, ●). Data are mean ± s.d. ( n = 5 per culture). (D) Specific growth rate ( μ ) during 24–120 h. Data are mean ± s.d. *: p < 0.01 (Tukey’s test, n = 5 per culture). (E) Representative Live/Dead staining images: live cells (Calcein-AM, green) and dead cells <t>(Propidium</t> iodide, PI, red). Scale bar: 500 μm. (F) Distribution of aggregate projected area over 0.3 × 10 5 μm 2 at t = 120 h. (G) Aggregate number over 0.3 × 10 5 μm 2 at t = 120 h. Data are mean ± s.d. *: p < 0.01 (Student’s t-test, n = 5 per culture). (H) Confocal fluorescence images of aggregate sections cultured with FITC-labelled chitin (green) only or with California Red-labelled chitosan (red) at t = 72 and 120 h using a 30-mL bioreactor at 50 rpm. Blue shows nuclei (DAPI). Scale bar: 100 μm.
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    Dynamic suspension culture using mixed of chitosan and chitin nanofiber scaffolds. Comparison of agitation conditions in the presence of chitosan/chitin (80:20) using a 30-mL bioreactor. (A) Specific growth rate ( μ ) during 24–96 h. Data are mean ± s.d.; no significant difference was detected (Tukey’s test, n = 3 per culture). (B) Distribution of aggregate projected area at t = 96 h. Comparison of scaffold conditions at 50 rpm in a 30-mL bioreactor. (C) Growth profiles under without scaffolds (open triangle, △), chitin only (open circle, ○), and chitosan/chitin (80:20) (closed circle, ●). Data are mean ± s.d. ( n = 5 per culture). (D) Specific growth rate ( μ ) during 24–120 h. Data are mean ± s.d. *: p < 0.01 (Tukey’s test, n = 5 per culture). (E) Representative Live/Dead staining images: live cells (Calcein-AM, green) and dead cells <t>(Propidium</t> iodide, PI, red). Scale bar: 500 μm. (F) Distribution of aggregate projected area over 0.3 × 10 5 μm 2 at t = 120 h. (G) Aggregate number over 0.3 × 10 5 μm 2 at t = 120 h. Data are mean ± s.d. *: p < 0.01 (Student’s t-test, n = 5 per culture). (H) Confocal fluorescence images of aggregate sections cultured with FITC-labelled chitin (green) only or with California Red-labelled chitosan (red) at t = 72 and 120 h using a 30-mL bioreactor at 50 rpm. Blue shows nuclei (DAPI). Scale bar: 100 μm.
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    Dynamic suspension culture using mixed of chitosan and chitin nanofiber scaffolds. Comparison of agitation conditions in the presence of chitosan/chitin (80:20) using a 30-mL bioreactor. (A) Specific growth rate ( μ ) during 24–96 h. Data are mean ± s.d.; no significant difference was detected (Tukey’s test, n = 3 per culture). (B) Distribution of aggregate projected area at t = 96 h. Comparison of scaffold conditions at 50 rpm in a 30-mL bioreactor. (C) Growth profiles under without scaffolds (open triangle, △), chitin only (open circle, ○), and chitosan/chitin (80:20) (closed circle, ●). Data are mean ± s.d. ( n = 5 per culture). (D) Specific growth rate ( μ ) during 24–120 h. Data are mean ± s.d. *: p < 0.01 (Tukey’s test, n = 5 per culture). (E) Representative Live/Dead staining images: live cells (Calcein-AM, green) and dead cells <t>(Propidium</t> iodide, PI, red). Scale bar: 500 μm. (F) Distribution of aggregate projected area over 0.3 × 10 5 μm 2 at t = 120 h. (G) Aggregate number over 0.3 × 10 5 μm 2 at t = 120 h. Data are mean ± s.d. *: p < 0.01 (Student’s t-test, n = 5 per culture). (H) Confocal fluorescence images of aggregate sections cultured with FITC-labelled chitin (green) only or with California Red-labelled chitosan (red) at t = 72 and 120 h using a 30-mL bioreactor at 50 rpm. Blue shows nuclei (DAPI). Scale bar: 100 μm.
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    Image Search Results


    Dynamic suspension culture using mixed of chitosan and chitin nanofiber scaffolds. Comparison of agitation conditions in the presence of chitosan/chitin (80:20) using a 30-mL bioreactor. (A) Specific growth rate ( μ ) during 24–96 h. Data are mean ± s.d.; no significant difference was detected (Tukey’s test, n = 3 per culture). (B) Distribution of aggregate projected area at t = 96 h. Comparison of scaffold conditions at 50 rpm in a 30-mL bioreactor. (C) Growth profiles under without scaffolds (open triangle, △), chitin only (open circle, ○), and chitosan/chitin (80:20) (closed circle, ●). Data are mean ± s.d. ( n = 5 per culture). (D) Specific growth rate ( μ ) during 24–120 h. Data are mean ± s.d. *: p < 0.01 (Tukey’s test, n = 5 per culture). (E) Representative Live/Dead staining images: live cells (Calcein-AM, green) and dead cells (Propidium iodide, PI, red). Scale bar: 500 μm. (F) Distribution of aggregate projected area over 0.3 × 10 5 μm 2 at t = 120 h. (G) Aggregate number over 0.3 × 10 5 μm 2 at t = 120 h. Data are mean ± s.d. *: p < 0.01 (Student’s t-test, n = 5 per culture). (H) Confocal fluorescence images of aggregate sections cultured with FITC-labelled chitin (green) only or with California Red-labelled chitosan (red) at t = 72 and 120 h using a 30-mL bioreactor at 50 rpm. Blue shows nuclei (DAPI). Scale bar: 100 μm.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: A fluffy nanofiber scaffold-based microenvironment enables a simplified, biology-centric scale-up strategy for mesenchymal stem/stromal cell culture

    doi: 10.3389/fbioe.2026.1808384

    Figure Lengend Snippet: Dynamic suspension culture using mixed of chitosan and chitin nanofiber scaffolds. Comparison of agitation conditions in the presence of chitosan/chitin (80:20) using a 30-mL bioreactor. (A) Specific growth rate ( μ ) during 24–96 h. Data are mean ± s.d.; no significant difference was detected (Tukey’s test, n = 3 per culture). (B) Distribution of aggregate projected area at t = 96 h. Comparison of scaffold conditions at 50 rpm in a 30-mL bioreactor. (C) Growth profiles under without scaffolds (open triangle, △), chitin only (open circle, ○), and chitosan/chitin (80:20) (closed circle, ●). Data are mean ± s.d. ( n = 5 per culture). (D) Specific growth rate ( μ ) during 24–120 h. Data are mean ± s.d. *: p < 0.01 (Tukey’s test, n = 5 per culture). (E) Representative Live/Dead staining images: live cells (Calcein-AM, green) and dead cells (Propidium iodide, PI, red). Scale bar: 500 μm. (F) Distribution of aggregate projected area over 0.3 × 10 5 μm 2 at t = 120 h. (G) Aggregate number over 0.3 × 10 5 μm 2 at t = 120 h. Data are mean ± s.d. *: p < 0.01 (Student’s t-test, n = 5 per culture). (H) Confocal fluorescence images of aggregate sections cultured with FITC-labelled chitin (green) only or with California Red-labelled chitosan (red) at t = 72 and 120 h using a 30-mL bioreactor at 50 rpm. Blue shows nuclei (DAPI). Scale bar: 100 μm.

    Article Snippet: Sampled cells were rinsed with D-PBS(−) and stained with 1.0 μg/mL Calcein-AM (C326; DOJINDO) and 2.0 μg/mL Propidium iodide (PI) (P378; DOJINDO) in a multi-well plate for 15 min at 37 °C.

    Techniques: Suspension, Comparison, Staining, Fluorescence, Cell Culture